The National Institute of Biology (NIB) is a public non-profit organization performing basic and applied research in the fields of biology, biotechnical sciences and biotechnology, medicine and ecology. The Department of Biotechnology and Systems Biology encompasses three research teams which conduct research of microorganisms and viruses, plant-pathogen interactions and systems biology, and of genetically modified organisms. The department has an excellent know-how on development of molecular diagnostic protocols for laboratory and on-site detection of bacteria, viruses and viroids based on state-of-the-art techniques including real-time PCR, LAMP, digital droplet PCR, and deep-sequencing. NIB has extensive experience in purification and concentration of plant and human viruses from different organisms and environmental samples (e.g. water) and of virus-like particles produced in plants. They are also experienced in the use of deep sequencing for detection and characterization of novel and previously described pathogens.