Identification of two novel putative satellite RNAs with hammerhead structures in the virome of French and Spanish carrot samples
Bisola Mercy Babalola, Deborah Schönegger, Chantal Faure, Armelle Marais, Aurora Fraile, Fernando Garcia-Arenal, and Thierry Candresse. https://doi.org/10.1007/s00705-022-05538-z. Carrot virome analysis by high-throughput sequencing revealed two RNA molecules with properties of satellite RNAs and are homologous to the satellite RNA of cereal yellow dwarf virus-RPV. Satellite 1 is 298 nt long, while satellite 2 is 368 nt long. Their positive and negative genome strands contain hammerhead ribozymes similar to those found in other self-cleaving satellite RNAs. While both satellites were detected in Spanish carrot populations, satellite 2 was also found in France. The most likely helper virus for these two satellites is the polerovirus carrot red leaf virus.
- 07 February 2023
- Yolanda Hernando Saiz
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Carrot virome analysis using high-throughput sequencing revealed the presence of two RNA molecules with properties of satellite RNAs that are homologous to the satellite RNA of cereal yellow dwarf virus-RPV (CYDV-RPV). Satellite 1 is 298 nt long, while satellite 2 is 368 nt long. Their positive and negative genome strands contain hammerhead ribozymes similar to those found in other self-cleaving satellite RNAs. While both satellites were detected in Spanish carrot populations, only satellite 2 was found in French carrot populations. The most likely helper virus for these two satellites is carrot red leaf virus (CtRLV), which, like CYDV-RPV, is a polerovirus.
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