Cont-ID: Detection of samples cross-contamination in viral metagenomic data
Johan Rollin*1,2, Wei Rong*1 and Sébastien Massart1. 1. University of Liège, Gembloux Agro-Bio Tech, Plant Pathology Laboratory, 5030, Gembloux, Belgium 2. DNAVision, 6041, Gosselies, Belgium. *These authors contributed equally to this work. https://doi.org/10.1101/2023.01.23.525161 Conclusions: Cross-contamination between samples when detecting viruses using HTS can be monitored and highlighted by Cont-ID (provided an alien control is present). Cont-ID is based on a flexible methodology relying on the output of bioinformatics analyses of the sequencing reads and considering the contamination pattern specific to each batch of samples. The Cont-ID method is adaptable so that each laboratory can optimise it before its validation and routine use.