First reports of Apple luteovirus 1, Apple rubodvirus 1 and Apple hammerhead viroid infecting apples in Belgium
Fontdevila Pareta, N., Lateur, M., Steyer, S., Blouin, A.G. & Massart, S. (2022) First reports of Apple luteovirus 1, Apple rubodvirus 1 and Apple hammerhead viroid infecting apples in Belgium. New Dis Rep. 45, e12076. https://doi.org/10.1002/ndr2.12076 As part of a pilot study on germplasm collections, leaves were collected from six apple trees (Malus domestica) in the germplasm collection of the CRA-W in Belgium in June 2019 and June 2020. Double-stranded RNA (dsRNA) and total RNA were extracted from collected leaf tissue, and the extracted nucleic acids were submitted to high-throughput sequencing (HTS).
- 22 January 2023
- Yolanda Hernando Saiz
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After read assembly using SPAdes Genome Assembler, viral contigs belonging to apple rubodvirus 1 (ARWV-1), apple luteovirus 1 (ALV-1) and apple hammerhead viroid (AHVd) were identified by BLASTn against the GenBank nucleotide database. The detection of the 3 species was confirmed by RT-PC. This constitutes the first report of ARWV-1, ALV-1, and AHVd in Belgium
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